Undifferentiated human adipose-derived stromal/stem cells loaded onto wet-spun starch-polycaprolactone scaffolds enhance bone regeneration : nude mice calvarial defect in vivo study

摘要

The repair of large bony defects remains challengingin the clinical setting. Human adipose-derived stromal/stem cells (hASCs) have been reported to differentiate alongdifferent cell lineages, including the osteogenic. The objectiveof the present study was to assess the bone regenerationpotential of undifferentiated hASCs loaded in starchpolycaprolactone(SPCL) scaffolds, in a critical-sized nudemice calvarial defect. Human ASCs were isolated from lipoaspirateof five female donors, cryopreserved, and pooledtogether. Critical-sized (4 mm) calvarial defects were createdin the parietal bone of adult male nude mice. Defects wereeither left empty, treated with an SPCL scaffold alone, orSPCL scaffold with human ASCs. Histological analysis andMicro-CT imaging of the retrieved implants were performed.Improved new bone deposition and osseointegration wasobserved in SPCL loaded with hASC engrafted calvarialdefects as compared to control groups that showed littlehealing. Nondifferentiated human ASCs enhance ossificationof nonhealing nude mice calvarial defects, and wet-spunSPCL confirmed its suitability for bone tissue engineering.This study supports the potential translation for ASC usein the treatment of human skeletal defects.